Microbiology lab

🔬Understanding Gram Staining process🧪 Gram staining is an essential technique in microbiology, allowing us to classify bacteria into Gram-positive and Gram-negative. This simple yet powerful method helps in identifying bacterial cell walls based on their ability to retain crystal violet dye. Gram-positive bacteria appear purple, while Gram-negative bacteria take on a pink hue. Such differentiation is crucial for diagnosing infections and determining appropriate treatments.🧫🧬 Excited to…

You will be working with many pathogenic species of bacteria in the laboratory. Remember that bacteria are in the air as well as on the skin, the counter, and all objects and equipment that have not been sterilized. The most important tool for transferring cultures is the wire inoculating needle or loop. It can be quickly sterilized by heating it to red hot in a Bunsen burner flame.

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Gram-positive bacteria retain the crystal violet-iodine complex and stain purple, whereas gram-negative bacteria stain pink.

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Gram negative lactose fermenting on MacConkey

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Culture media nutrient agar notes study